Police are appealing for witnesses following a fatal road traffic collision near Glenrothes.
The incident took place at around 1.15pm on Saturday 26 January on the A911 Glenrothes to Windygates road, at West Mill Road known locally as Blue Bridge.
A grey Peugeot and blue Vauxhall travelling west were involved in a collision with a black Honda travelling east.
The 73-year-old female driver of the Peugeot was taken to the Edinburgh Royal Infirmary with serious injuries and sadly passed away in hospital on Sunday morning.
A 70-year-old female with serious injuries and 78-year-old man with minor injuries were taken to Ninewells Hospital, Dundee.
A 73-year-old female was taken to Victoria Hospital, Kirkcaldy with serious injuries.
The road was closed for approximately six hours for collision investigation at the scene.
Sergeant Ewan Pearce from Fife’s Road Policing Unit said: “Tragically as a result of the collision a lady has lost her life and our thoughts are with her family and friends at this difficult time.
“I would ask anyone who may have been travelling on the A911 at the time of the collision on Saturday afternoon and who witnessed what happened to come forward to assist our investigation.
“Anyone who may have dash-cam footage of the vehicles immediately prior to the collision, and has not yet spoken to officers, is asked to get in touch to provide this at their earliest opportunity.”
Those with information can contact Police Scotland on 101, quoting incident number 1958 of 26 January.